Instituto de Investigação e Inovação em Saúde, Universidade do Porto
Rua Alfredo Allen, 208 4200-135 Porto, Portugal
Phone: +351 226 074 900
Email: info@i3s.up.pt
Publications
Mateus JC*, Melo P, Aroso M, Charlot B, Aguiar P*. Influence of asymmetric microchannels in the structure and function of engineered neuronal circuits. Biofabrication. 2025; 17: 025022
Arede L, Coutinho R, Duarte TL, Lopes M, Simões RV, Silva S, Baptista P, Lopes JM, Faria R, Duarte D. Recapitulation of clinical features in a patient-derived xenograft mouse model of VEXAS syndrome. Rheumatology (Oxford). 2025; 64(9): 5189-5192.
Simoes RV, Henriques RN, Olesen JL, Cardoso BM, Fernandes FF, Monteiro MAV, Jespersen SN, Carvalho T, Shemesh N. Deuterium metabolic imaging phenotypes mouse glioblastoma heterogeneity through glucose turnover kinetics. Elife. 2025; 13: RP100570.
Bilreiro C, Fernandes FF, Simões RV, Henriques R, Chavarrías C, Ianus A, Castillo-Martin M, Carvalho T, Matos C, Shemesh N. Pancreatic Intraepithelial Neoplasia Revealed by Diffusion-Tensor MRI. Invest Radiol. 2025; 60(6): 397-406.
Sousa SC, Aroso M, Bessa R, Veríssimo E, da Silva TF, Lopes CD, Brites P, Vieira J, Vieira CP, Aguiar P, Sousa MM. “Stretch triggers microtubule stabilization and MARCKS-dependent membrane incorporation in the shaft of embryonic axons”. Current Biology. 2024 Oct 7;34(19):4577-88.
Horton S, Mastrolia V, Jackson RE, Kemlo S, Machado PM, Carbajal MA, Hindges R, Fleck RA, Aguiar P, Neves G, Burrone J. “Excitatory and inhibitory synapses show a tight subcellular correlation that weakens over development”. Cell Reports. 2024 Jul 23;43(7).
Oliveira AM, Carvalho E, Barros B, Soares C, Ferreira-Pinto MJ, Vaz R, Aguiar P*. “DBScope as a versatile computational toolbox for the visualization and analysis of sensing data from deep brain stimulation”. npj Parkinson’s Disease. 2024 Jul 15;10(1):132.
Mateus JC, Sousa MM, Burrone J, Aguiar P*. “Beyond a Transmission Cable—New Technologies to Reveal the Richness in Axonal Electrophysiology”. Journal of Neuroscience. 2024 Mar 13;44(11).
Castro DL, Aroso M, Aguiar AP, Grayden DB, Aguiar P*. “Disrupting abnormal neuronal oscillations with adaptive delayed feedback control”. Elife. 2024 Mar 7;13:e89151.
Oliveira AM, Coelho L, Carvalho E, Ferreira-Pinto MJ, Vaz R, Aguiar P*. “Machine learning for adaptive deep brain stimulation in Parkinson’s disease: closing the loop”. Journal of Neurology. 2023 Nov;270(11):5313-26.
Manubens-Gil L, Zhou Z, Chen H, Ramanathan A, Liu X, Liu Y, et al, Aguiar P, et al, Peng H. “BigNeuron: a resource to benchmark and predict performance of algorithms for automated tracing of neurons in light microscopy datasets”. Nature Methods. 2023 Apr 17:1-2.
Miranda J, Paules C, Noell G, Youssef L, Paternina-Caicedo A, Crovetto F, Cañellas N, Garcia-Martín ML, Amigó N, Eixarch E, Faner R, Figueras F, Simões RV, Crispi F, Gratacós E. Similarity network fusion to identify phenotypes of small-for-gestational-age fetuses. iScience. 2023; 26(9): 107620.
Dias C, Castro D, Aroso M, Ventura J, Aguiar P*. “Memristor-Based Neuromodulation Device for Real-Time Monitoring and Adaptive Control of Neuronal Populations”. ACS Applied Electronic Materials. 2022 May 2;4(5):2380-7.
Mateus JC, Weaver S, van Swaay D, Renz AF, Hengsteler J, Aguiar P, Vörös J. “Nanoscale patterning of in vitro neuronal circuits”. ACS Nano. 2022 Apr 11;16(4):5731-42.
Neto E, Leitão L, Mateus JC, Sousa DM, Alves CJ, Aroso M, Monteiro AC, Conceição F, Oreffo RO, West J, Aguiar P, Lamghari M. “Osteoclast-derived extracellular vesicles are implicated in sensory neurons sprouting through the activation of epidermal growth factor signaling”. Cell & bioscience. 2022 Aug 14;12(1):127.
Dantas M, Oliveira A, Aguiar P, Maiato H, Ferreira JG. “Nuclear tension controls mitotic entry by regulating cyclin B1 nuclear translocation”. Journal of Cell Biology. 2022 Oct 12;221(12):e202205051.
Conde-Sousa E, Vale J, Feng M, Xu K, Wang Y, Della Mea V, La Barbera D, et al, Aguiar P, Eloy C, Polónia A. “HEROHE Challenge: Predicting HER2 Status in Breast Cancer from Hematoxylin–Eosin Whole-Slide Imaging”. Journal of Imaging. 2022 Jul 31;8(8):213.
Almeida AC, Soares-de-Oliveira J, Drpic D, Cheeseman LP, Damas J, Lewin HA, Larkin DM, Aguiar P, Pereira AJ, Maiato H. “Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals”. Cell Reports. 2022 Apr 5;39(1):110610.
Muñoz-Moreno E, Simões RV, Tudela R, López-Gil X, Soria G. Spatio-temporal metabolic rewiring in the brain of TgF344-AD rat model of Alzheimer’s disease. Sci Rep. 2022; 12(1): 16958.
Mateus JC, Lopes CD, Aroso M, Costa AR, Gerós A, Meneses J, Faria P, Neto E, Lamghari M, Sousa MM, Aguiar P*. “Bidirectional flow of action potentials in axons drives activity dynamics in neuronal cultures”. Journal of Neural Engineering. 2021 Dec 29;18(6):066045
Horta R, Frias F, Barreiro D, Gerós A, Aguiar P. “Dynamic Facial Reanimation in an Overweight Patient and with Significant Comorbidities: An Objective Analysis of Labbè Technique”. The Surgery Journal. 2021 Oct;7(04):e342-6.
Blenkuš U, Gerós AF, Carpinteiro C, Aguiar P, Olsson IA, Franco NH. “Non-Invasive Assessment of Mild Stress-Induced Hyperthermia by Infrared Thermography in Laboratory Mice”. Animals. 2022 Jan;12(2):177
Polónia A, Campelos S, Ribeiro A, Aymore I, Pinto D, Biskup-Fruzynska M, Veiga RS, Canas-Marques R, et al, Aguiar P, Eloy C. “Artificial intelligence improves the accuracy in histologic classification of breast lesions”. American Journal of Clinical Pathology. 2021 Apr;155(4):527-36.
Teixeira H, Dias C, Aguiar P, Ventura J, “Gold‐Mushroom Microelectrode Arrays and the Quest for Intracellular‐Like Recordings: Perspectives and Outlooks”, (2021) Advanced Materials Technologies, 2000770
Castro D, Nunes V, Lima J, Ferreira J, Aguiar P*, ”Trackosome: a computational toolbox to study the spatiotemporal dynamics of centrosomes, nuclear envelope and cellular membrane”, (2020), Journal of Cell Science, 133
Costa AR, Sousa SC, Pinto-Costa R, et al, Aguiar P, Sousa M, “The membrane periodic skeleton is an actomyosin network that regulates axonal diameter and conduction”, (2020) eLife 9, e55471
Nunes V, Dantas M, Castro D, Vitiello E, Wang I, Carpi N, Balland M, Piel M, Aguiar P, Maiato H, Ferreira JG. “Centrosome-nuclear axis repositioning drives the assembly of a bipolar spindle scaffold to ensure mitotic fidelity”, (2020) Molecular Biology of the Cell, 2020 Jul 21:mbc-E20
Gerós A, Magalhães A, Aguiar P*, “Improved 3D tracking and automatic classification of rodent’s behavioral activity using depth-sensing cameras”, (2020) Behavior Research Methods, 1-12
Mateus JC, Lopes CDF, Cerquido M, Leitão L, Leitao DC, Cardoso S, Ventura J, Aguiar P*. “Improved in vitro electrophysiology using 3D-structured microelectrode arrays with a micro-mushrooms islets architecture capable of promoting topotaxis”, (2019) J Neural Engineering, 16-036012
Heiney K, Mateus JC, Lopes CDF, Neto E, Lamghari M, Aguiar P*, “uSpikeHunter: An advanced computational tool for the analysis of neuronal communication and action potential propagation in microfluidic platforms”, (2019), Scientific Reports, 9-5777
Aresta G, Araújo T, Kwok S, Chennamsetty SS, Safwan M, et al, Aguiar P*, “BACH: Grand challenge on breast cancer histology images”, (2019) Medical image analysis, 56:122-39
Franco NH, Gerós A, Oliveira L, Olsson IAS, Aguiar P*, “ThermoLabAnimal – A high-throughput analysis software for non-invasive thermal assessment of laboratory mice”, (2019) Physiology & Behavior 207, 113-121
Loureiro J, Torres AL, Neto T, Aguiar P, Barrias CC, Pinto MT, Amaral IF, “Conjugation of the T1 sequence from CCN1 to fibrin hydrogels for therapeutic vascularization”, (2019) Materials Science and Engineering: C, 104, 109847
Torres AL, Bidarra SJ, Pinto MT, Aguiar P, Silva EA, Barrias CC, “Guiding morphogenesis in cell-instructive microgels for therapeutic angiogenesis”, (2018) Biomaterials, 154:34-47
Leitão L, Alves CJ, Alencastre IS, Sousa DM, Neto E, Conceição F, Leitão C, Aguiar P, Almeida-Porada G, Lamghari M, “Bone marrow cell response after injury and during early stage of regeneration is independent of the tissue-of-injury in 2 injury models”, (2018) The FASEB Journal, fj-201800610RR.
Maiato H, Drpic D, Almeida A, Aguiar P, Renda F, Damas J, Lewin H, Larkin D, Khodjakov A, ”How kinetochore size affects chromosome segregation”, (2018) FEBS OPEN BIO, 8:19-19.
Lopes CD, Mateus JC, Aguiar P*, “Interfacing Microfluidics with Microelectrode Arrays for Studying Neuronal Communication and Axonal Signal Propagation”, (2018) JoVE, 142:e58878.
Cerquido M, Proenca MP, Dias C, Leitao DC, Cardoso S, Freitas PP, Aguiar P, Ventura J, “Tailoring the cap’s morphology of electrodeposited gold micro-mushrooms”, (2018) Applied Surface Science, 445:512-8.
Drpic D, Almeida AC, Aguiar P, Renda F, Damas J, Lewin HA, Larkin DM, Khodjakov A, Maiato H, “Chromosome segregation is biased by kinetochore size”, (2018) Current Biology, 28(9):1344-56.
Fondón I, Sarmiento A, García AI, Silvestre M, Eloy C, Polónia A, Aguiar P, “Automatic classification of tissue malignancy for breast carcinoma diagnosis”, (2018) Computers in Biology and Medicine, 96:41-51.
Horta R, Nascimento R, Gerós A, Aguiar P, Silva A, Amarante J, “A Novel System for Assessing Facial Muscle Movements: The Facegram 3D”, (2018) Surgical Innovation, 25(1):90-2.
Dias C, Lv H, Picos R, Aguiar P, Cardoso S, Freitas PP, Ventura J, “Bipolar resistive switching in Si/Ag nanostructures.”, (2017) Applied Surface Science, DOI: 10.1016/j.apsusc.2017.01.140
Martins LF, Costa RO, Pedro JR, Aguiar P, Serra SC, Teixeira FG, Sousa N, Salgado AJ, Almeida RD, “Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF”, (2017), Scientific Reports, 7(1):4153.
Mestre AL, Cerquido M, Inácio PM, Asgarifar S, Lourenço AS, Cristiano ML, Aguiar P, Medeiros MC, Araújo IM, Ventura J, Gomes HL, “Ultrasensitive gold micro-structured electrodes enabling the detection of extra-cellular long-lasting potentials in astrocytes populations”, (2017) Scientific Reports, 7(1):14284.
Mestre AL, Inácio P, Elamine Y, Asgarifar S, Lourenço AS, Cristiano ML, Aguiar P, Medeiros MC, Araújo IM, Ventura J, Gomes HL, “Extracellular Electrophysiological Measurements of Cooperative Signals in Astrocytes Populations”, (2017), Frontiers in neural circuits, 11:80.
Araujo T, Aresta G, Castro E, Rouco J, Aguiar P, Eloy C, Polonia A, Campilho A, “Classification of breast cancer histology images using convolutional neural networks”, (2017) PLoS ONE, 12(6):e0177544
Shamarova E, Chertovskih R, Ramos AF, Aguiar P*, “Backward-stochastic-differential-equation approach to modeling of gene expression”, (2017) Phys. Rev. E, 95, 032418
Conde-Sousa E, Szücs P, Peng H, Aguiar P*, “N3DFix: An algorithm for automatic removal of swelling artifacts in neuronal reconstructions.”, (2017) Neuroinformatics, 15(1):5-12
Lopes CD, Gomes CP, Neto E, Sampaio P, Aguiar P, Pêgo AP, “Microfluidic-based platform to mimic the in vivo peripheral administration of neurotropic nanoparticles”, (2016) Nanomedicine, 11(24):3205-3221
Neto E, Leitão L, Sousa D, Alves CJ, Alencastre IS, Aguiar P, Lamghari M, “Compartmentalized microfluidic platforms – the unrivaled refresh of in vitro tools for neurobiological research”, (2016) J Neuroscience, 36(46):11573-11584
Pereira CL, Teixeira GQ, Ribeiro-Machado C, Caldeira J, Costa M, Figueiredo F, Fernandes R, Aguiar P, Grad S, Barbosa MA, Gonçalves RM, “Mesenchymal Stem/Stromal Cells seeded on cartilaginous endplates promote Intervertebral Disc Regeneration through Extracellular Matrix Remodeling”, (2016) Scientific Reports, 6,33836
Sousa DM, Conceição F, Silva D, Leitão L, Neto E, Alves CJ, Alencastre IS, Herzog H, Aguiar P, Lamghari M, “Ablation of Y1 receptor impairs osteoclast bone-resorbing activity”, (2016) Scientific Reports, 6,33470
Teixeira G, Pereira C, Castro F, Ferreira J, Lázaro M, Aguiar P, Wilke H, Ignatius A, Barbosa M, Neidlinger-Wilke C, Gonçalves R, “Anti-inflammatory Chitosan/Poly-ɣ-glutamic acid nanoparticles control inflammation while remodeling extracellular matrix in degenerated intervertebral disc”, (2016) Acta Biomaterialia, 42:168-179
Gerós A, Horta R, Aguiar P*, “Facegram – Objective quantitative analysis in facial reconstructive surgery”, (2016) J Biomedical Informatics, 61:1-9
Pereira AJ, Aguiar P, Belsley M, Maiato H, “Inducible fluorescent speckle microscopy”, (2016) Journal of Cell Biology, 212 (2), 245-255
Alencastre I, Sousa D, Alves C, Leitão L, Neto E, Aguiar P, Lamghari M, “Delivery of pharmaceutics to bone: Nanotechnologies, High-throughput processing and in silico mathematical models.”, (2016) European Cells and Materials Journal, 31: 355-381
Dias C, Guerra M, Ventura J*, Aguiar P*, “Memristor-based Willshaw network: capacity and robustness to noise in the presence of defects”, (2015) Applied Physics Letters, 106, 223505
Silva , Duarte R, Sampaio J, Aguiar P, Davids K, Araujo D, Garganta J, “Field dimension and skill level constrain team tactical behaviours in small-sided and conditioned games in football”, (2014) Journal of Sports Sciences, 32(20), pp 1888-1896
Barisic M, Aguiar P, Geley S and Maiato H, “Kinetochore motors drive congression of peripheral polar chromosomes by overcoming random arm-ejection forces”, (2014) Nature Cell Biology, 16(12):1249-56
Horta R, Aguiar P, Monteiro D, Silva A, Amarante JM, “A facegram for spatial-temporal analysis of facial excursion: Applicability in the microsurgical reanimation of long-standing paralysis and pretransplantation”, (2014) Journal of Cranio-Maxillofacial Surgery, 42(7), 1250–1259
Silva P, Travassos B, Vilar L, Aguiar P, Davids K, Araújo D, Garganta J, “Numerical relations and skill level constrain co-adaptive behaviors of agents in sports teams”, (2014) PLOS ONE, 9 (9)
Sousa M, Szucs P, Lima D and Aguiar P*, “The pronociceptive dorsal reticular nucleus contains mostly tonic neurons and frequently shows spontaneous activity without ascending input from the spinal cord”, (2014) J Neurophysiology, 111 (7), pp 1507-1518
Horta R, Aguiar P, Silva P, Monteiro D, Amarante JM, “Facial disfigurement: Restoration of facial dynamics in a patient with concomitant facial paralysis and blindness”, (2014) Journal of Reconstructive Microsurgery, 30 (3), pp. 207-210
Sousa M, Aguiar P*, “Building, simulating and visualizing large spiking neural networks with NeuralSyns”, (2014) Neurocomputing, 123, pp 372-380
Castro L, Aguiar P*, “A feedforward model for the formation of a grid field where spatial information is provided solely from place cells”, (2014) Biological Cybernetics, 108 (2), pp. 133-143
Afonso O, Matos I, Pereira A, Aguiar P, Lampson A, Maiato H, “Feedback control of chromosome separation by a midzone Aurora B gradient”, (2014) Science, 345 (6194), pp 332-336
Silva P, Aguiar P, Duarte R, Davids K, Araújo D, Garganta J, “Effects of pitch size and skill level on tactical behaviours of association football players during small-sided and conditioned games”, (2014) Intl J of Sports Science and Coaching, 9(5), pp 993-1006
Neto E, Alves CJ, Sousa DM, Alencastre IS, Lourenço AH, Leitão L, Ryu HR, Jeon NL, Fernandes R, Aguiar P, Almeida RD, Lamghari M, “Sensory neurons and osteoblasts: close partners in a microfluidic platform”, (2014) Integrative Biology (Camb), 6(6), pp 586-595
Moutinho-Pereira S, Stuurman N, Afonso O, Hornsveld M, Aguiar P, Goshima G, Vale RD, Maiato H, “Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells”, (2013) PNAS – Proceedings of the National Academy of Sciences of the United States of America, 110 (49), pp. 19808-19813
Guimarães J, Moura E, Silva E, Aguiar P, Garrett C, Vieira-Coelho MA, “Locus coeruleus is involved in weight loss in a rat model of Parkinson’s disease: An effect reversed by deep brain stimulation”, (2013) Brain Stimulation, 6 (6), pp. 845-855
Conde-Sousa E, Aguiar P*, “A working memory model for serial order that stores information in the intrinsic excitability properties of neurons”, (2013) Journal of Computational Neuroscience, 35 (2), pp. 187-199
Aguiar P*, Sousa M, Szucs P, “Versatile morphometric analysis and visualization of the three-dimensional structure of neurons”, (2013) Neuroinformatics, 11 (4), pp. 393-403
Horta R, Monteiro D, Silva A, Amarante JM, Aguiar P*, “Evaluation of a facial transplant candidate with a facegram: A baseline analysis”, (Plastic and Reconstructive Surgery (2013) 132 (479e-480)), (2013) Plastic and Reconstructive Surgery, 132 (4), pp. 1040-1041
Silva P, Garganta J, Araújo D, Davids K, Aguiar P, “Shared knowledge or shared affordances? Insights from an ecological dynamics approach to team coordination in sports”, (2013) Sports Medicine, 43 (9), pp. 765-772
Szucs P, Luz LL, Pinho R, Aguiar P, Antal Z, Tiong SYX, Todd AJ, Safronov BV, “Axon diversity of lamina I local-circuit neurons in the lumbar spinal cord”, (2013) Journal of Comparative Neurology, 521 (12), pp. 2719-2741
Carneiro J, Duarte-Pereira S, Azevedo L, Castro LFC, Aguiar P, Moreira IS, Amorim A, Silva RM, “The Evolutionary Portrait of Metazoan NAD Salvage”, (2013) PLoS ONE, 8 (5), art. no. e64674
Pinto S, Krishna R, Dias C, Pimentel G, Oliveira GNP, Teixeira JM, Aguiar P, Titus E, Gracio J, Ventura J, Araujo JP, “Resistive switching and activity-dependent modifications in Ni-doped graphene oxide thin films”, (2012) Applied Physics Letters, 101 (6), art. no. 063104
Castro L, Aguiar P*, “Phase precession through acceleration of local theta rhythm: A biophysical model for the interaction between place cells and local inhibitory neurons”, (2012) Journal of Computational Neuroscience, 33 (1), pp. 141-150
Pais-Vieira M, Aguiar P, Lima D, Galhardo V, “Inflammatory pain disrupts the orbitofrontal neuronal activity and risk-assessment performance in a rodent decision-making task”, (2012) Pain, 153 (8), pp. 1625-1635
Aguiar P*, Sousa M, Lima D, “NMDA channels together with L-type calcium currents and calcium-activated nonspecific cationic currents are sufficient to generate windup in WDR neurons”, (2010) Journal of Neurophysiology, 104 (2), pp. 1155-1166
Aguiar P*, Mendonça L, Galhardo V, “OpenControl: A free opensource software for video tracking and automated control of behavioral mazes”, (2007) Journal of Neuroscience Methods, 166 (1), pp. 66-72
Vourvouhaki E, Carvalho C, Aguiar P, “Model for Osteosarcoma-9 as a potent factor in cell survival and resistance to apoptosis”, (2007) Physical Review E – Statistical, Nonlinear, and Soft Matter Physics, 76 (1), art. no. 011926
Aguiar P, Willshaw D, “Hippocampal mossy fibre boutons as dynamical synapses”, (2004) Neurocomputing, 58-60, pp. 699-703